Kinetic Studies on Yeast Growth (part 5)

Kinetic studies of yeast hexokinase.

The mechanism of calf brain hexokinase has recently been studied in our laboratory (1). It was concluded from kinetic experiments that adenosine diphosphate (ADP) dissociates from the enzyme before addition of substrate glucose. This hypothesis was supported by kinetic studies of n-mannose, ADP, and glucose g-phosphate inhibition. The experiments appear to lend credence to theories in which an ...

Studies of the kinetic mechanism of orotate phosphoribosyltransferase from yeast.

Kinetic analysis of the reaction catalyzed by a homogeneous preparation of orotate phosphoribosyltransferase from yeast has revealed that the reaction may proceed through the use of a Bi Bi Ping Pong kinetic mechanism. This interpretation is based on initial rate measurements and product inhibition studies of both the forward phosphoribosyl transfer and the reverse pyrophosphorolysis reactions ...

Studies on Growth Acceleration in Protozoa and Yeast

Studies on Yeast Zymin

In previous communications by the authors (1935) data were presented on the effect of some electrolytes, and of ethanol, upon the evolution of carbon dioxide from glucose by yeast aymin. The electrolytes, in appropriate concentrations, stimulate the rate of evolution of the gas. The zymin is very sensitive to ethanol. The addition of phosphate does not decrease the sensitivity toward ethanol, w...

Studies on Yeast Metabolism

Direct measurement ofthe metabolic changes in the intracellular carbohydrate ofyeast has usually been restricted to the determination oftotal hydrolysable carbohydrate, that is to say, ofreducing power after acid hydrolysis of a cell suspension. The work of Pickett & Clifton (1943), or of Fales & Baumberger (1948), exemplifies this technique applied respectively to the aerobic and anaerobic met...